Biostatistics and Bioinformatics

Paper Code: 
MBL 125
Credits: 
05
Contact Hours: 
75
Objective: 

to enable students to-

  • Appreciate the significance of statistical analysis of biological data.
  • Learn the methods of statistical analysis.

know about Bioinformatics as a tool in Biotechnology

11.00
Unit I: 
Sampling and classification of data

Sampling - Sampling procedure, types of sampling, Classification and tabulation of data, frequency distribution, probability, addition and multiplication theorem of probability.

A brief idea of normal, Poisson and binomial distribution.

 

 

14.00
Unit II: 
Measures of central tendency and dispersion

Measure of central tendency-Mean, median and mode, Measures of dispersion - range , mean deviation ,standard deviation, coefficient of variation, Skewness and kurtosis.

 

16.00
Unit III: 
Tests of Significance

Hypothesis testing, Nulls hypothesis and alternative hypothesis, level of significance. Chi-square test, t-test, F-test, ANOVA-one way and two way classifications. Simple correlation and simple regression.

 

15.00
Unit IV: 
Bioinformatics

Overview of bioinformatics – introduction, the internet and the biologist, Database types-Primary and Secondary databases, sequence databases - nucleotide and protein sequence databases (NCBI, EMBL, DDBJ, UNIPORT, PIR), Structural databases (PDB, MMDB, CSD, NDB)

Genomics and Genome project (a brief idea)

 

19.00
Unit V: 
Sequence analysis

Concept of similarity searching, methods of similarity searching (BLAST, FASTA) statistical significance of sequence comparisons, application of similarity searching in gene identification and functional assignment. Information retrieval from biological databases.Computer tools for finding and retrieving sequences, pair wise and multiple alignments.

ESSENTIAL READINGS: 

1.      Biostatistics: A Foundation for Analysis in Health Sciences, (6th edition), W W Daniel, John Wiley and Sons Inc., 1995.

2.      Essential Bioinformatics, Jin Xiong, John Wiley and Sons. 2006.

3.      Introduction to Bioinformatics, A Teresa and D P Smith, Prentice Hall, 1999.

4.      Statistical Methods in Biology, N T J Bailey, Cambridge University Press, 1995.

5.     Statistics for Biologist, R C Campbell, Cambridge University Press, 1989

REFERENCES: 
  1. Bioinformatics, A practical Guide to the Analysis of Genes and Proteins, (2nd edition), A D Baxevanis, and B F Ouellette, John Wiley and Sons, 2002.
  2. Fundamentals of Biostatistics, Khan,  Publishing Corporation, 1999
  3. Instant notes, Bioinformatics, Westhead, Parish, and Twyman, (1st edition), Bios Scientific Publishers Ltd., 2003.
  4. Introduction to Bioinformatics, A M Lesk, Oxford University Press, 2002. 
  5. Molecular databases for Protein sequence and Structure studies, J A Sillince and M  Sillince, Springer Verlag, 1991
  6. Practical statistics for Experimental Biologists, A C Swardlaw,  John Wiley and sons Inc., 1985
  7. Sequence Analysis Primer, Gribskov and Devereux, Stockton Press, 1989

 

Academic Year: 
2014-2015
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