MOLECULAR BIOLOGY

Paper Code: 
BTE 302
Credits: 
3
Contact Hours: 
45
Objective: 
  • understand the basic processes involved in the flow of genetic information
  • study the processing of the protein synthesized
  • understand the regulation of gene expression
8.00
Unit I: 
DNA: The genetic material

 

DNA and Genome: The primary structure of DNA, packaging of DNA, Gene in molecular terms, physical and chemical nature of nucleic acid,types of DNA (A, B, Z).

12.00
Unit II: 
DNA replication and recombination

DNA replication in prokaryotes and eukaryotes, enzymes involved in replication, DNA repair mechanism- mismatch repair, base excision repair, Nucleotide excision repair photoreactivation, Recombination and repair (Holiday model)

10.00
Unit III: 
Transcription and Post-transcriptional modifications

Transcription in prokaryotes and eukaryotes, mRNA structure (coding and non coding RNA; miRNA, siRNA and hnRNA), Ribozyme, Posttranscriptional modifications. 

8.00
Unit IV: 
Translation and Post-translational modifications

 

Genetic code, Prokaryotic and eukaryotic translation, Post translational modification of proteins and its breakdown, inhibitors of protein synthesis.

8.00
Unit V: 
Regulation of gene expression

 

Regulation of gene expression in prokaryotes and eukaryotes at transcription and translation level, Operon concept-positive and negative regulation of lac operon, trp operon

ESSENTIAL READINGS: 

 

1.     Molecular Biology and Biotechnology, H.D. Kumar, 2nd revised version, Vikas Publishing House, 1996

2.     Principles of Genetics, 8th edition, Gardner, Simmons, Snustad, 2003

3.     Genomes by T.A. Brown, Bios Scientific Publishers Ltd., 1999

REFERENCES: 

1.     Cell and Molecular Biology- Concepts and Experiments, 4th edition, Gerald Karp, Willey International Edition, 2005

2.     Molecular Biology, Instant Notes, 2nd edition, P.C. Turner, A.G. Mc Lennan, A.D. Bates and M.R.H. White, Viva Books Pvt. Ltd., 2002

3.     Biochemistry and Molecular Biology- William H. Elliot, Daphne C. Elliot, Oxford University Press, 2005.

4.     Molecular Genetics, 2nd edition, An Introductory Narrative, Gunther S. Stent, Richard Calendar

5.     Essentials of Molecular Biology, 3rd edition by Malacinski and Freifelder, Jones and Bartlet Publishers, 1998

6.     Nucleic Acids by Shawn Doonan, University of East, London, 2004.

7.     Principles and Technique of Biochemistry and Molecular Biology, 6th edition, Keith Wilson and John Walker, Cambridge University Press, 2007.

Academic Year: 
2015-2016
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