LABORATORY I

1. Microtomy of liver/intestine
2. Histochemical localistion of starch, lipids and protein in liver.
3. Verification of Beer Lambert’s Law
4. Estimation of protein by Folin Lowry method
5. Estimation of protein by Biuret test
6. Estimation of reducing sugar by DNSA method
7. Estimation of sugar by Anthrone reagent
8. Estimation of glycogen from the given tissue
9. Estimation of cholesterol from the given tissue
10. To study the effect of substrate concentration on enzyme activity
11. To study the effect of temperature on enzyme activity
12. To study the effect of pH on enzyme activity
13. To assay the activity of acid phosphatase
14. Separation and identification of amino acids by TLC
15. Separation of protein by SDS-PAGE
16. Demonstrations:
    
    1.  Gel Filtration Chromatography
    2. Ion Exchange Chromatography
    3. Descending Paper Chromatography
    4. Column Chromatography
    5. Affinity Chromatography
    6. Western Blotting
17. Preparation of liquid and solid media for growth of microorganisms.
18. Isolation and maintenance of organisms by plating, streaking and serial dilution methods, slants and stab cultures, storage of microorganisms.
19. Isolation of pure cultures from soil and water.
20. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on growth.
21. Enumeration by standard plate count.
22. Microscopic examination of bacteria, yeast and molds and study of organisms by Gram stain, Negative staining, staining for spores etc.
23. Isolation of Rhizobia
24. Isolation of Actinomycetes
25. Biochemical characterization of selected microbes.
26. Biostatistics exercises
27. Operating systems (DOS)
28. Information searching on Internet.
29. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ

Any other practical based on theory syllabus.