LABORATORY II

1. Study of mitosis and meiosis.
2. X – Chromosome detection through Barr body experiment.
3. To study mitosis in onion root tips
4. To study meiosis in onion buds
5. Isolation of plasmid DNA 
6. Isolation of genomic DNA
7. Spectrophotometric quantitation of  nucleic acids
8. Demonstration of lac operon
9. Blood film preparation and identification of cells.
10. Ouchterlony double diffusion
11. Dot ELISA
12. Demonstrations:
    
    1. Sandwich ELISA
    2. Latex agglutination
    3. Rocket Immunoelectrophoresis
13. Restriction digestion of λDNA
14. Ligation
15. Transformation of DH5α (including preparation of competent cells)
16. Demonstartions:
    
    1. Conjugation
    2. Reporter gene assay (GFP)
17. Preparation of media.
18. Explant inoculation
19. Callus induction
20. Protoplast isolation and fusion
21. Synthetic seed preparation
22. Study of mutagens by Ames test
23. Blood Film preparation and identification of cells

Any other practical based on theory syllabus