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BIOTECHNOLOGY AND BIOINFORMATICS
Principles and methods of recombinant DNA technology.,
cloning, sequencing, polymerase chain reaction,cloning in E.coli, plasmids, bacteriophages and cosmid vectors, cloning strategies, genomic and cDNA library; cloning in yeast: transformation in yeast, yeast vector development: Yep, YRp, YCp and YIp, 2m plasmid, yeast artificial chromosome (YAC), retrovirus like vector (Ty) in east/shuttle vector; features of yeast promoter and expression of cloned genes; yeast 2-hybrid system; plasmid shuffling to explore interactive domains of multimeric proteins; the cassette model for mating type switches and silencing of genes.
Antisense RNA, Antisense oligonucleotides ,RNAi in plants and fungi, application of RNAi for studying gene expression,functional screening with RNAi libraries. Nanobiotechnology
Identifying new genes with metagenomics, culture enrichment for environmental samples, sequence-dependent techniques for metagenomics, Function or activity-based evaluation of the environment. Ecology and metagenomics, Natural attenuation of pollutants
Molecular databases- primary and secondary databases,Sequence alignment techniques, scoring systems – dynamic programming, similarity searching using BLAST and FASTA. EMBOSS tools for DNA and protein sequence analysis
MSA and phylogenetic analysis, methods of phylogenetic tree construction, Bootstrapping technique protein structure analysis and modelling
Books Recommended:
1. Plant, Gene and crop Biotechnology, M.J. Chrispeel and D.E. Sadava ASPB 2003.
2. Textbook of Environmental Biotechnology, P.K Mohapatra, I K International
Publications, Mumbai 2006.
3. Environmental Chemistry, A.K.De, Willey Eastern Ltd., New Delhi.
4. Microbial Ecology- Fundamentals and applications. Ronald. M. Atlas, Richard
Bartha. Pearson Education. 2005.
5. Advances in Industrial Waste water treatment. P.K. Goel. (First Edition).
Technosound Publications. 1999.
6. Wastewater Engineering- Treatment, Disposal and Resuse, Metcalf and Eddy,
Inc., Tata McGraw Hill, Delhi.1999
7. Comprehensive Biotechnology, vol.4, M. Moo- Young (Ed-in-chief), Pergamon
Press, Oxford.
8. Introduction to Biodeterioration. D. Allsopp and K.J. Seal, ELBS/Edward Arnold.
9. Bioremediation Engineering: design and Application. Cookson, J.T. McGraw-
Hill, Inc.1995
10. Environmental Biotechnology – Basic concepts and applications, Indu Shekhar
Thakur, I K International publications. 2006 Molecular Cloning: a Laboratory
11. Manual, J. Sambrook. E.F. Fritsch and T. Maniatis, Cold Spring Harbor
Laboratory Press, New York, 2000
12. DNA Cloning: a practical Approach, D. M. Glover and B.D. Hames, IRL Press
Oxford, 1955.
13. Molecular and Cellular Methods in Biology and Medicine, P. B. Kaufman, W.
Wu., D. Kim and L.J: Cseke, CRC Press.Florida,1995.
14. An Introduction to Genetic Engineering. Edited by Desmond S.T. Nicholl,
Cambridge University Press, February 2002.
15. Manipulation and Expression of Recombinant DNA. Sue Carson and Dominique
16. Robertson, Second edition, Academic Press, December 2005.
17. Principles of Gene Manipulation and Genomics. Primrose and Twyman, (7th
edition). Blackwell Publishers, 2006.
18. RNA interference Technology- From basic science to drug development. Edited by Krishnarao Appasani. Forewords by Andrew Fire and Marshall Nirenberg,Cambridge Press, 2005.
19. Methods in Enzymology Vol.152,Guide to Molecular Cloning Techniques, S. L.
Berger and A. R. Kimmel, Academic press. Inc. San Dlogo, 1998.
20. Methods in Enzymology Vol.185,Gene Expression Technology, D. V. Gooddol, Academic Press, Inc., San Diego,1990.
21. DNA Science. A First Course in Recombinant Technology, D.A. Mickloss and G.A. Greyer, Cold Spring Harbor Laboratory Press, New York,1990
22. Molecular Biotechnology (2nd Edition), S.B. Primorse, Blackwell Scientific
Publishers, Oxford, 1994.
23. Milestones in Biotechnology. Classic papers on genetic Engineering, J.A. Davies and W.S.Roznikolf, Butterworth-Helnemann, Boston, 1992.
24. Route Maps in Gene Technology, M. R. Walker and R.Repley, Blackwell Science Ltd. Oxford, 1997.
25. Genetic Engineering. An Introduction to gene analysis and exploitation in
Eukaryotes. S. M. Kingsman and A.J. Kingsman, Blackwell Scientific Publications. Oxford, 1998.
