FERMENTATION TECHNOLOGY AND RECOMBINANT DNA TECHNOLOGY

Paper Code: 
DMBL 501A
Credits: 
04
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO 39A: Correlate designing and working of various fermenters

CO 40A: Analyze medium formulation, inoculum preparation and sterilization

CO 41A: Formulate recovery operations together with the fundamental principles for basic methods in production technique for bio-based products.

CO 42A: Acquaint and plan tools for genetic engineering and use various types of vectors

CO 43A: Apply various Methods of gene transfer in research.

 

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

14.00
Unit I: 
Bioreactors and Fermentation Process

Bioreactors:  Basic Design, Conventional and Specialized bioreactors (pulsed, fluidized, photo bioreactors), Types of fermentation processes batch, fed batch and continuous cultures. Primary and Secondary screening techniques.

 

12.00
Unit II: 
Media Production and Control of Fermentation Parameters

Raw materials used in media production, inoculum preparation, industrial sterilization, Probes for process monitoring and control of fermentation parameters (temperature, pH, dissolved oxygen, foam).

 

10.00
Unit III: 
Principles and Operation of Downstream Processing

Cell disruption, filtration, centrifugation, liquid-liquid extraction, solvent extraction (distillation), preparative ion exchange chromatography, drying.

 

12.00
Unit IV: 
Recombinant DNA Technology-I

Gene cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC; Enzymes used in gene manipulation- Restriction endonucleases, DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase.

 

12.00
Unit V: 
Recombinant DNA Technology- II

Methods of gene transfer, Making and applications of genomic and c-DNA libraries; Blotting Techniques- Southern, Northern and Western Blotting.

    

 

ESSENTIAL READINGS: 
  • Principles of Fermentation Technology by P. Stanbury, A. Whitaker, S. Hall.     3rd edition. Butterworth-Heinemann. 2016.
  • Fermentation Microbiology and Biotechnology edited by E.M.T. El-Mansi, C.F. Bryce, A.L. Demain, A.R. Allman. 3rd edition. CRC Press. 2012.
  • Bioprocess Engineering: Basic Concepts by M. L. Shuler, F. Kargi, 2 nd edition. Pearson Education India. 2015.
  • Brown, T. A., Gene Cloning and DNA Analysis, An Introduction.  Wiley –Blackwell publication. 2010.

 

 

REFERENCES: 

SUGGESTED READINGS:

  • Roger G. Harrison, Paul W. Todd, Scott R. Rudge, Demetri P. Petrides, Bioseparations Science and Engineering, 2nd Ed., Oxford University Press, 2003.
  • Pauline M. Doran, Bioprocess Engineering Principles, 2nd Ed., Academic Press, 2012
  • James Bailey, David Ollis, Biochemical Engineering Fundamentals, 2nd Ed., McGraw Hill Education, 2017.
  • Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.

 

e RESOURCES:

 

JOURNALS:

  • Journal of Fermentation Technology
  • Journal of Industrial Microbiology and Biotechnology
  • International Journal of Molecular Biotechnology
  • Journal of Fermentation and Bioengineering

 

 

 

 

 

 

 

 

 

 

 

 

 

Academic Year: 
2023-2024
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