Fundamentals of Biochemistry

Paper Code: 
MBL 101
Credits: 
3
Contact Hours: 
45
Objective: 

·      The nature of biological material and hierarchy of molecular organization of cells

·      The thermodynamic principles underlying biochemical reactions

·      The function of macromolecules

·      The reaction mechanism and regulation of enzyme activity

10.00
Unit I: 
Nature of Biomolecules and Thermodynamics

 Cell:  Chemical components, Molecular Hierarchy

Types of interactions in molecular and supramolecular complexes

Laws of thermodynamics, Nature of biochemical reactions and DG values, Reversible and Irreversible reactions

Water and the aqueous environment

Buffers: Concept of pH, pK, acids, bases

 

7.00
Unit II: 
Carbohydrates

 

Structure of monosaccharide (trioses, pentoses & hexoses), disaccharides (sucrose, maltose, lactose), polysaccharides (starch, glycogen, cellulose, peptidoglycan)

Fundamental Properties: Stereoisomerism and mutarotation

                             

11.00
Unit III: 
Nucleic Acids & Proteins

 

Biochemical structure of nucleic acid

Biological functions of Nucleic Acids. The central Dogma and its exceptions. Structure of essential and non-essential amino acids

Peptide bond, structural hierarchy of protein

Protein classification on the basis of structure and function                                           

Biological functions of proteins

7.00
Unit IV: 
Lipids

                                                                                        

Structure and nomenclature of fatty acids

Classification of lipids (triacylglycerols, phospholipids, sphingolipids, sterols)

Membrane lipids

Biological functions of lipids

 

10.00
Unit V: 
Enzymes

                                                                             

Classification and nomenclature of enzymes                                                                   

      Mechanism of enzyme action, Michaelis- Menton kinetics

      Coenzymes, Enzyme inhibition, Allosteric enzymes

Regulation of enzyme activity

ESSENTIAL READINGS: 
  1. Biochemistry, 2nd edition, D Voet and J G Voet, John Wiley & Sons, 1995.
  2. Lehninger, 3rd edition, Principles of Biochemistry, Nelson and Cox, 1999.
  3. Principles and Techniques in Biochemistry and Molecular Biology, 6th edition, Edited by Keith Wilson and John Walker, CMBLridge University Press, 2007.
  4. Biochemistry and Molecular Biology, 3rd Edition, W H Elliot and Daphne C Elliot, Oxford University Press, 2005.
  5. Biochemistry, J David Rawn, Panima Publishing Corporation, 1st Indian Reprint, 2004.
  6. Biochemistry, 5th edition L Stryer, W H Freeman and Co., New York, 2001

REFERENCES: 

 

  1. Physical Biochemistry, D Friefelder, W.H. Freeman & Company, 17th Reprint, 1999.
  2. Understanding Chemistry, CNR Rao, Universities Press, Hyderabad 1999.
  3. A Biologist's Guide to Principles and Techniques of Practical Biochemistry, K Wilson & K H Goulding, ELBS Edition 1986.
  4. Essentials of Molecular Biology, David Friefelder, Jones and Barllett Publications, 7th Reprint, 2001.
  5. Encyclopedia of Molecular Biology, J Kendrew, Blackwell Scientific Publications, Oxford.

<>6.Instant Notes- Biochemistry, B D Hames and N.M. Hooper, Viva Books Pvt, Ltd., 1st Indian Edition, 2001.

Academic Year: 
2015-2016
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