Fundamentals of Biochemistry

Paper Code: 
MBL 101
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to understand –

1. The nature of biological material and hierarchy of molecular organization of cells.

2. The thermodynamic principles underlying biochemical reactions and the function of macromolecules.

3. The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

 

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Asses sment Strate

gies

Paper Code

Paper Title

 

 

 

 

 

 

 

 

 

 

 

MBL 101

 

 

 

 

 

 

 

 

 

 

Funda mental s of Bioche mistry

CO 6

Understand the concepts of electrolytes    and                   electrolytic dissociation,      pH      and      its biological significance, buffers, Henderson.-Hasselbalch equation,                   biological                    buffer systems and their importance. CO 7

Understand the laws of thermodynamics , concepts of entropy, enthalpy and free energy changes and their application to biological systems and various biochemical studies and reactions.

CO 8

Overview of major biomolecules

–carbohydrates, lipids, proteins, aminoacids, nucleic acids, classification.

CO 9

Structure, function of the above mentioned biomolecules

CO 10

Conceptual knowledge of properties, structure, function of enzymes, enzyme kinetics

and their regulation

Approach                   in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students: Self          learning assignments, Effective questions, Seminar presentation, Giving tasks.

C       A

test, Semes ter end examin ations, Quiz, Assign ments, Classro om interac tion

 

10.00
Unit I: 
Nature of Biomolecules and Thermodynamics
  • Cell: Chemical components, Molecular Hierarchy
  • Types of interactions in molecular and supramolecular complexes
  • Laws of thermodynamics, Nature of biochemical reactions and DG values, Reversible and Irreversible reactions
  • Water and the aqueous environment
  • Buffers: Concept of pH, pK, acids, bases

 

7.00
Unit II: 
Carbohydrates
  • Structure of monosaccharide (trioses, pentoses & hexoses), disaccharides (sucrose, maltose, lactose), polysaccharides (starch, glycogen, cellulose, peptidoglycan)
  • Fundamental Properties: Stereoisomerism and mutarotation

 

 

11.00
Unit III: 
Nucleic Acids & Proteins
  • Biochemical structure of nucleic acid
  • Biological functions of Nucleic Acids. The central Dogma and its exceptions. Structure of essential and non-essential amino acids
  • Peptide bond, structural hierarchy of protein
  • Protein classification on the basis of structure and function
  • Biological functions of proteins

 

7.00
Unit IV: 
Lipids
  • Structure and nomenclature of fatty acids
  • Classification of lipids (triacylglycerols, phospholipids, sphingolipids, sterols)
  • Membrane lipids
  • Biological functions of lipids

 

10.00
Unit V: 
Enzymes
  • Classification and nomenclature of enzymes
  • Mechanism of enzyme action, Michaelis- Menton kinetics
  • Coenzymes, Enzyme inhibition, Allosteric enzymes
  • Regulation of enzyme activity

 

 

ESSENTIAL READINGS: 
  • Biochemistry, 2nd edition, D Voet and J G Voet, John Wiley & Sons, 1995.
  • Lehninger, 3rd edition, Principles of Biochemistry, Nelson and Cox, 1999.
  • Principles and Techniques in Biochemistry and Molecular Biology, 6th edition, Edited by Keith Wilson and John Walker, CMBLridge University Press, 2007.
  • Biochemistry and Molecular Biology, 3rd Edition, W H Elliot and Daphne C Elliot, Oxford University Press, 2005.
  • Biochemistry, J David Rawn, Panima Publishing Corporation, 1st Indian Reprint, 2004.
  • Biochemistry, 5th edition L Stryer, W H Freeman and Co., New York, 2001
  • Physical Biochemistry, D Friefelder, W.H. Freeman & Company, 17th Reprint, 1999.
  • Understanding Chemistry, CNR Rao, Universities Press, Hyderabad 1999.
  • A Biologist's Guide to Principles and Techniques of Practical Biochemistry, K Wilson & K H Goulding, ELBS Edition 1986.
  • Essentials of Molecular Biology, David Friefelder, Jones and Barllett Publications, 7th Reprint, 2001.
  • Encyclopedia of Molecular Biology, J Kendrew, Blackwell Scientific Publications, Oxford.
  • Instant Notes- Biochemistry, B D Hames and N.M. Hooper, Viva Books Pvt, Ltd., 1st Indian Edition, 2001.

 

Academic Year: 
2021-2022
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