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GENETICS AND MOLECULAR BIOLOGY
Course Outcomes (COs):
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO 10: Illustrate the different types of gene interactions and predict the outcomes of different genetic crosses. CO 11: Compare and contrast the different methods of recombination in prokaryotes CO 12: Analyse the molecular basis of mutations CO 13: Understand extrachromosomal inheritance and Hardy Weinberg equilibrium. CO 14: Illustrate prokaryotic and eukaryotic gene structure and function CO 15: Compare and contrast the mechanisms of bacterial and eukaryotic DNA replication CO 16: Correlate gene regulation in prokaryotes and eukaryotes CO 17: Analyze translation mechanism in prokaryotes and eukaryotes and regulation of gene expression |
Interactive Lectures, Discussion, Tutorials, Demonstrations, Assignments Reading Journals |
Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects |
Mendelian genetics-Mendel’s Experiment and Laws of inheritance, allelic and non-allelic interactions-co dominance, incomplete dominance, Epistasis (recessive and dominant), Complementary and Supplementary genes, duplicate and lethal genes. Sex determination mechanisms (XX-XY, ZZ-ZW and Haplodiploidy), Non-disjunction and Genetic balance theory, Dosage compensation, Sex linked inheritance.
Extra chromosomal inheritance, Maternal inheritance, uniparental inheritance and maternal effect Linkage, Recombination in bacteria-conjugation, transformation and transduction
Spontaneous and induced mutations, types of point mutations, physical and chemical mutagens. DNA repair mechanism- mismatch repair, base excision repair, Nucleotide excision repair, photoreactivation, Structural and numerical chromosomal aberrations. Hereditary defects in humans-Downs syndrome, Klinfelters syndrome and Turners syndrome.
DNA and Genome: The primary structure and packaging of DNA, Gene in molecular terms, physical and chemical nature of nucleic acid, types of DNA (A, B, Z). DNA replication inprokaryotes and eukaryotes, enzymes involved in replication, Recombination and repair (Holiday model).
Transcription in prokaryotes and eukaryotes, mRNA structure and synthesis.Ribozyme, Post-transcriptional modifications. Prokaryotic and eukaryotic translation, Post-translational modification of proteins and its breakdown.Regulation in prokaryotes and eukaryotes at genomic, transcription and translation level, Operon concept-positive and negative regulation of lac operon, trp operon.
- Principles of Genetics, (12th edition), E J Gardener, M J Simmons and D P Snustead, John Wiley and Sons Publications, 2012
- Introduction to genetic analysis, Griffiths, A., Wessler, S., Lewontin, R., Carroll, S. 9th edition, W. H. Freeman and Company, 2007.
- Genetics, (3rd edition), M V Strickberger, New Delhi : PHI Learning, 2012
- Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York,2012.
- Genomes. Brown T.A: Published by Garland Science Publishing, a member of the Taylor & Francis Group, 2002
- Molecular Biology of the Cell (6th Edition) Bruce Albert, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter, 2014
- Molecular Biology of the Gene (7th Edition) Watson, Baker, Bell, Gann, Levin, Losick: Pearson Education.2017.
- Molecular Cell Biology (9th Edition) J. Darnell, H. Lodish and D.Baltimore, Macmillan learning, 2021.
- Freifelder’s Essentials of Molecular Biology. Malacinski, G M, Jones & Bartlett Publishers Inc. (2015)
SUGGESTED READINGS:
- Cell Biology & Molecular Biology, 8th edition, E D P Roberties and E M F Roberties, Lippincott Williams and Wilkins, 2017
- Principles of genetics, D Peter Snustad; Michael J Simmons, 7th edition, Hoboken, NJ: John Wiley & Sons, Inc., 2017
- From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003
- Genetics – A molecular approach by Russell, Peter. J, 2016.
- Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.
- Lewin’s Gene XII, Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick, 12th edition Jones and Bartlett Publishers, Inc, 2017
e RESOURCES:
- Hake, L. & O'Connor, C. (2008) Genetic mechanisms of sex determination. Nature Education 1(1):25
- Saccone, G., Pane, A., &Polito, L. C. Sex determination in flies, fruit flies and butterflies. Genetica 116, 15–23 (2002)
- Miko, I. (2008) Gregor Mendel and the principles of inheritance. Nature Education 1(1):134
- Clancy, S. (2008) Genetic mutation. Nature Education 1(1):18
- Andrews, C. (2010) The Hardy-Weinberg Principle. Nature Education Knowledge 3(10):65
- https://doi.org/10.1016/B978-0-12-374984-0.00996-7
- https://open.umn.edu/opentextbooks/textbooks/244
- https://www.web-books.com/MoBio/Free/Chap3.htm
- https://www.web-books.com/MoBio/Free/Chap4.htm
- https://www.web-books.com/MoBio/Free/Chap5.htm
- https://www.web-books.com/MoBio/Free/Chap6.htm
- https://www.web-books.com/MoBio/Free/Chap7.htm
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JOURNALS:
- Journal of Genetics
- Frontiers in Genetics
- Annual Reviews: Biomedical Sciences (Annual Reviews/HighWire Press)
Cell (Cell Press/HighWire Press) - EMBO Journal (Oxford University Press/HighWire Press)
- Nature (Macmillan Publishers)
- PNAS (National Acedemy of Sciences/HighWire Press)
- Science (AAAS/Highwire Press)
- https://onlinelibrary.wiley.com/doi/book/10.1002/9781118015759
