INDUSTRIAL MICROBIOLOGY ANDRECOMBINANT DNA TECHNOLOGY

Paper Code: 
DMBL 501B
Credits: 
04
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

  
 

Upon completion of the course the learner will:

CO 39 B: Articulate principles and methods of bioprocesses in industrial fermentations

CO 40 B: Apply microbial processes in industrial fermentations

CO 41 B: Illustrate the various applications of microbes in Industries.

CO 42 B: Acquaint and plan tools for genetic engineering and use various types of vectors

CO 43 B: Apply various Methods of gene transfer in research.

Interactive Lectures, Discussion, Tutorials, Reading assignments, Self-learning assignments, Effective questions, Giving tasks

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

  

 

 

14.00
Unit I: 
Bioreactors and fermentation processes

Bioreactors:  Basic Design, Types of fermentation processes batch, fed batch and continuous cultures. Screening techniques, Raw materials used in media production, inoculum preparation, industrial sterilization.

 

12.00
Unit II: 
Industrial fermentations

Industrial Production of chemicals utilizing wastes: Alcohol (ethanol) acids (citric), antibiotics (Penicillin), Amino acids (Gutamic acid), Enzymes (Amylase, Lipase).            

10.00
Unit III: 
Industrial application of microbes

Biomass based products: Biopesticides (Thuricide and Trichoderma), Yeast: SCP, Baker’s and Distiller’s yeast. Microbial transformation products: Steroids, Mushroom cultivation

 

12.00
Unit IV: 
Gene manipulation techniques- I

Gene cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC; Enzymes used in gene manipulation- Restriction endonucleases, DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase.

 

12.00
Unit V: 
Recombinant DNA Technology- II

Methods of gene transfer, Making and applications of genomic and c-DNA libraries; Blotting Techniques- Southern, Northern and Western Blotting.

 

ESSENTIAL READINGS: 
  • Industrial Microbiology, E Casida, New Age International (P) Ltd. Publishers. 2005
  • Manual of Industrial Microbiology and Biotechnology, A L Demain and J E Davies, ASM Press, 1999
  • Principles of Fermentation Technology, 2nd edition, P F Stanbory, Whitaker and Hall, Elsevier India, 2005
  • Gene IX, Lewin, Oxford university Press, 2007
  • Microbial Genetics, 2nd edition, Maloy, D Freidfelder and John E  Cronan., Jones and Barlett Publishers, 1994
  • Principles of Genetics, 8th edition, E J Gardener, M J Simmons and D P Snustad, John Wiley and Sons Publications, 2005
  • Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology. 2nd Edition. Panima Publishing Company, New Delhi
  • Patel AH. (1996). Industrial Microbiology .1st Edition. MacMillan India Limited Publishing Company Ltd. New Delhi, India
  • Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd edition, Elsevier Science Ltd
  • Brown, T. A., Gene Cloning and DNA Analysis, An Introduction.  Wiley –Blackwell publication. 2010.

 

REFERENCES: 

SUGGESTED READINGS:

  • A Textbook of Industrial Microbiology, 2nd edition, W Crueger and A Crueger, Panima Publication Corporation, 2000
  • Fermentation Microbiology and Biotechnology, Mansi and Bryce, Taylor & Francis group, 2002
  • Industrial Microbiology, 4th edition, S C Prescott and D C Dunn, CBS Publishers and Distributors, 1987
  • Nanobiotechnology: Concepts, Applications & Perspectives, C N Niemeyer and
  • C A Mirkin, Wiley-VCH Verlag GmbH & Co., 2004
  • Process Biotechnology Fundamentals, 2nd edition, S N Mukhopadhyaay, Viva books Pvt. Ltd., 2004
  • Fermentation Microbiology and Biotechnology. E. M. T. El-Mansi (Editor), C. F. A. Bryce (Editor), Arnold L. Demain (Editor). CRC Press. 2012.
  • Demain, A. L and Davies, J. E. (1999). Manual of Industrial Microbiology and Biotechnology, 2nd Edition, ASM Press
  • Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.

 

e RESOURCES:

 

 

JOURNALS:

  • Enzyme and Microbial Technology
  • Microbial technology
  • Journal of Fermentation Technology
  • The Journal of Industrial Microbiology
  • Journal of Industrial Microbiology and Biotechnology
  • International Journal of Molecular Biotechnology

 

 

 

Academic Year: 
2023-2024
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