INSTRUMENTATION

Paper Code: 
MBL 124
Credits: 
05
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

 

                   Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learner will:

CO 18: Acquire knowledge of Bioinstrumentation

CO 19: Understand the laws of Spectroscopy and basic principles of Spectroscopic techniques.

CO 20: Employ analytical resasoning in biological sample separation through Centrifugation techniques

CO 21: Apply scientific knowledge to address research problems using electrophoretic separation techniques.

CO 22: Employ scientific reasoning in analyzing and addressing practical problemsin Chromatography

CO 23: Acquire the ability to engage in research and development using Microscopic analysis

Interactive Lectures, Discussion, Tutorials, Reading assignments

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

 
 
15.00
Unit I: 
Spectroscopy
  • Beer-Lambert law, UV-Vis spectroscopy, fluorescence spectroscopy, IR spectroscopy, Raman spectroscopy, Atomic absorption spectroscopy, Mass spectroscopy, NMR, ESR, CD/ORD, MALDI-ToF-MS, X-ray diffraction.
15.00
Unit II: 
Chromatography
  • Principle, Paper chromatography, TLC, Column chromatography (GC, HPLC), Adsorption chromatography, Partition chromatography, Gel filtration, Ion-exchange chromatography and Affinity chromatography.
  • Special note on GCMS/LCMS
15.00
Unit III: 
Electrophoresis
  • Principle, factors affecting electrophoresis, pH, voltage, supporting medium (agarose, polyacrylamide, dextran).
  • Agarose gel electrophoresis, PAGE, SDS-PAGE, 2-D electrophoresis, Pulsed field gel electrophoresis, isoelectric focusing, immuno electrophoresis.
15.00
Unit IV: 
Centrifugation & Radioisotopes
  • Centrifugation: principle differential and density gradient, ultracentrifugation (Preparative and analytical centrifuges) sedimentation analysis & RCF.
  • Radioisotopes; nature of radioactivity, types of radioactive decay, unit of radioactivity.
  • Autoradiography, Cerenckov radiation, liquid scintillation counting, Geiger-Muller counter.
15.00
Unit V: 
Microscopy

·         Microscopy: construction of a microscope, light microscopy (bright field, dark field, phase-contrast, interference, confocal, polarization microscopy).

·         Electron microscopy-TEM, SEM, Scanned probe microscopic techniques (STEM, AFM)

Cytophotometry, flow cytometry. Cryo-electron microscopy.

ESSENTIAL READINGS: 
  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2007.
  • Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc., 2009
  • Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2008
  • Principles and Techniques of Biochemistry and Molecular Biology, (7th edition), K Wilson and J Walker (editor), Cambridge University Press, 2010
REFERENCES: 

SUGGESTED READINGS:

  • Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2014
  • Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005  

 

e RESOURCES:

 

JOURNALS:

  • Indian Journal of Experimental Biology
  • Journal of Scientific and Industrial Research
Academic Year: 
2022-2023
  • Printer-friendly version
  • PDF version