INSTRUMENTATION

Paper Code: 
MBL 124
Credits: 
5
Contact Hours: 
75
Objective: 

to enable students to-

  • Understand the basic operating characteristics of instruments.
  • Understand principles and applications of techniques.
17.00
Unit I: 
Spectroscopy

Beer-Lambert law, UV-Vis spectroscopy, fluorescence spectroscopy, IR spectroscopy, Raman spectroscopy, Atomic absorption spectroscopy, Mass spectroscopy, NMR, ESR, CD/ORD, MALDI-ToF-MS, X-Ray Diffraction.

15.00
Unit II: 
Chromatography

Principle, Paper chromatography, TLC, Column chromatography (GC, HPLC), Adsorption chromatography, Partition chromatography, Gel filtration, Ion-exchange chromatography and Affinity chromatography

15.00
Unit III: 
Electrophoresis

Principle, factors affecting electrophoresis, pH, voltage, supporting medium (agarose, polyacrylamide, dextran).

Agarose gel electrophoresis, PAGE, SDS-PAGE, 2-D electrophoresis, Pulsed field gel electrophoresis, isoelectric focusing, immuno electrophoresis.

20.00
Unit IV: 
Centrifugation & Microscopy

Centrifugation: principle differential and density gradient, ultracentrifugation (Preparative and analytical centrifuges) sedimentation analysis & RCF.

Microscopy: construction of a microscope, light microscopy (bright field, dark field, phase-contrast, interference, confocal, polarization microscopy).

Electron microscopy-TEM, SEM, Scanned probe microscopic techniques (STEM, AFM)

Cytophotometry, flow cytometry.                                                                                                                

8.00
Unit V: 
Radioisotopic Techniques

Radioisotopes; nature of radioactivity, types of radioactive decay, unit of radioactivity.

Radiation dosimetry, radioactive isotopes, autoradiography, Cerenckov radiation, liquid scintillation counting, Geiger-Muller counter.

ESSENTIAL READINGS: 
  1. Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
  2. Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou,  Fitzgerald science press, Inc., 1998
  3. Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003
  4. Principles and Techniques  of Biochemistry and  Molecular Biology ,(6th edition), K Wilson and J Walker (editor), Cambridge University Press, 2007
REFERENCES: 
  1. Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004    
  2. Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
  3. Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2004
  4. Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005 
Academic Year: 
2016-2017
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