LABORATORY –I
Objective:
- Verification of Beer’s law
- Quantitation of proteins by Lowry’s method
- Quantitation of proteins by Biuret assay
- Quantitation of carbohydrates by DNS method
- Quantitation of cholesterol
- Quantitation of lipids
- Separation of given amino acid mixture by paper chromatography
- Separation of sugars by thin layer chromatography
- Separation of the components of the given sample by gel filtration chromatography- Demonstration
- Qualitative test for carbohydrates, lipids, and proteins