LABORATORY –III

Paper Code: 
MBL 303
Credits: 
02
Contact Hours: 
60
Objective: 

This paper will enable students to understand –

 

  1. Learn the principle and working of different instruments.
  2. Learn good laboratory practices.
  3. Qualitative and quantitative estimation of various biomolecules.

 

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 303

Laboratory III

CO 1SStudent will learn to culture and identify protozoa.

CO 2SStudent will learn to isolate and genomic DNA, Plasmid DNA as well as RNA.

CO 3SStudent will learn various fungal staining techniques.

CO 4SStudent will learn to isolate and Enumerate various fungi.

CO 5SStudent will earn pure culture techniques for fungi.

 

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

 

CONTENTS

  1. Isolation of antibiotic resistant bacteria
  2. Isolation of genomic DNA
  3. Isolation of plasmid DNA
  4. Isolation of RNA
  5. Isolation of fungi from soil
  6. Identification of fungi by lactophenol cotton blue staining
  7. Enumeration of   fungal spores by haemocytometer
  8. Identification of Algae (permanent slides)
  9. Identification of protozoa (permanent slides)
  10. Preparation of protozoan culture
  11. Studies of live protozoa and preparation of slides
Academic Year: 
2020-2021
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