LABORATORY I

This course will enable the students to –

1. Make the students learn the practical aspect of Bacteriology, Biochemistry, Enzymology, Microbial Physiology and Diversity,
2. Gain practical knowledge on Instrumentation, biostatistics and bioinformatics

Course Outcomes (COs):

CONTENTS

1. Preparation of liquid and solid media for growth of microorganisms.
2. Pure culture techniques- streak plate method, pour plate method, spread plate method.
3. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on growth.
4. Enumeration of bacteria: standard plate count.
5. Microscopic examination of bacteria by simple staining, Gram stain, Negative staining, staining for spores etc.
6. Hanging drop method to study motility in bacteria.
7. study of culture characters of microorganisms
8. Biochemical characterization of selected microbes.

1. oxidation and fermentation tests
2. catalase test
3. oxidase test
4. nitrate reduction test
5. starch hydrolysis
6. IMVIC
7. H₂S production test

9. Estimation of protein in bacteria by Folin Lowry method
10. Estimation of protein by Biuret test
11. Estimation of reducing sugar by DNSA method
12. Estimation of sugar in bacteria by Anthrone reagent
13. To study the effect of substrate concentration on enzyme activity
14. To study the effect of temperature on enzyme activity
15. To study the effect of pH on enzyme activity
16. To assay the activity of acid phosphatase
17. Separation and identification of amino acids by TLC
18. Separation of protein by SDS-PAGE
19. Demonstrations:
    
    1. Gel Filtration Chromatography
    2. Ion Exchange Chromatography
    3. Adsorbtion  Chromatography
    4. Affinity Chromatography
    5. Blotting Techniques
20. Biostatistics exercises
21. Information searching on Internet.
22. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ

Any other practical based on theory syllabus.