Laboratory I

Paper Code: 
MBL 103
Credits: 
02
Contact Hours: 
60
Objective: 
  1. Learn the principle and working of different instruments.
  2. Learn good laboratory practices.
  3. Qualitative and quantitative estimation of various biomolecules.

 

Course Outcomes (COs):

 

 

Course

Learning      outcome (at course level)

Learning and teaching strategies

Assessme nt Strategies

Paper Code

Pape

r Title

 

 

CO 15

Approach    in

C A   test,

 

 

Understand the layout

teaching:

Semester

 

 

of      a      microbiology

Interactive

end

 

 

laboratory.

Lectures,

examinatio

 

 

CO 16

Discussion,

ns,       Viva

 

 

To get acquainted with

Practical

Voce,

 

 

the various instruments in      a     microbiology/

experiments

Practical record

 

 

biochemistry laboratory

Learning

book,

 

 

CO 17

activities for

Assignment

MBL 103

Labo

rator y I

Understand        various

accessories              for microbiology practical

the students:

Self learning assignments,

s,

Classroom interaction

 

 

CO 18

Effective

 

 

 

To    make    qualitative

questions,

 

 

 

and            quantitative

Practical

 

 

 

description of the basic

experiments

 

 

 

enzymatic phenomena

in lab, Giving

 

 

 

and processes.

CO 19

tasks.

 

 

 

Cultivate bacteria with

 

 

 

 

different        cultivation

 

 

 

 

technique

 

 

 

CONTENTS

  1. Preparation of buffers
  2. To determine the pH of solutions.
  3. To prepare molar and normal solutions
  4. Verification of Beer’s law
  5. Quantitation of proteins by Biuret assay
  6. Quantitation of carbohydrates by DNS method
  7. Quantitation of cholesterol
  8. Separation of given amino acid mixture by paper chromatography
  9. Separation of sugars by thin layer chromatography
  10. To study the working of light microscope and centrifuge

 

Academic Year: