LABORATORY I

Paper Code: 
BTE 126
Credits: 
8
Contact Hours: 
240
Objective: 

Course Outcomes (COs):

 

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO 30:  Evaluate the different types of aseptic methods.

CO 31: Apply the knowledge gained from different staining methods in study of morphology of bacteria, Gram’s nature and presence of endospores.

CO 32: Design and formulate the media for isolation of bacteria using pure culture techniques.

CO 33: Design an experiment to enumerate the no. of CFU/ml of any sample.

CO 34:   Analyze the bacterial growth by spectrophotometer.

CO 35: Categorize and apply different chromatographic techniques as well as learn and remember membrane transport methods.

CO  36: Relate, predict, illustrate practical aspect of Enzymology, Biochemistry and microbial growth and diversity

CO 37: Design application aspect of Instrumentation, biostatistics and bioinformatics

Hands-on practical, Interactive Lectures,

Discussion,

Demonstrations, Simulations

Class test, Semester end examinations, Hands-on practical assessment

 
 

 

 

  1. Microtomy of liver/intestine
  2. Histochemical localistion of starch, lipids and protein in liver.
  3. Verification of Beer Lambert’s Law
  4. Estimation of protein by Folin Lowry method
  5. Estimation of protein by Biuret test
  6. Estimation of reducing sugar by DNSA method
  7. Estimation of sugar by Anthrone reagent
  8. Estimation of glycogen from the given tissue
  9. Estimation of cholesterol from the given tissue
  10. To study the effect of substrate concentration on enzyme activity
  11. To study the effect of temperature on enzyme activity
  12. To study the effect of pH on enzyme activity
  13. To assay the activity of acid phosphatase
  14. Separation and identification of amino acids by TLC
  15. Separation of protein by SDS-PAGE
  16. Demonstrations:
    • Gel Filtration Chromatography
    • Ion Exchange Chromatography
    • Descending Paper Chromatography
    • Column Chromatography
    • Affinity Chromatography
    • Western Blotting
  17. Preparation of liquid and solid media for growth of microorganisms.
  18. Isolation and maintenance of organisms by plating, streaking and serial dilution methods, slants and stab cultures, storage of microorganisms.
  19. Isolation of pure cultures from soil and water.
  20. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on growth.
  21. Enumeration by standard plate count.
  22. Microscopic examination of bacteria, yeast and molds and study of organisms by Gram stain, Negative staining, staining for spores etc.
  23. Isolation of Rhizobia
  24. Isolation of Actinomycetes
  25. Biochemical characterization of selected microbes.
  26. Biostatistics exercises
  27. Operating systems (DOS)
  28. Information searching on Internet.
  29. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ
  30. Any other practical based on theory syllabus.

 

Academic Year: 
2022-2023
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