LABORATORY I

Paper Code: 
MBL 103
Credits: 
03
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learners will be able to:

CO 13: remember and learn good laboratory practices 

CO 14: acquire the ability to explain principle and working of different instruments and analytical techniques.

CO 15: employ scientific basics to solution preparation and pH estimation.

CO 16: to analyze and evaluate quantitative estimation of various biomolecules using spectroscopy.

Interactive Lectures, Discussion, Practical experiments, Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 
 

CONTENTS

  1. Preparation of buffers
  2. To determine the pH of solutions.
  3. To prepare molar and normal solutions
  4. Verification of Beer’s law
  5. Quantitation of proteins by Biuret assay
  6. Quantitation of carbohydrates by DNS method
  7. Quantitation of cholesterol
  8. Separation of given amino acid mixture by paper chromatography
  9. Separation of sugars by thin layer chromatography
  10. To study the working of light microscope and centrifuge

 

 

Academic Year: 
2022-2023
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