LABORATORY I

Paper Code: 
MBL 126
Credits: 
08
Contact Hours: 
240
Objective: 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course the learner will:

CO 31: Estimate different biomolecules using spectrophotometric method

CO 32: Assess enzyme activity

CO 33: Learn Hands on experience on Chromatography, Electrophoresis and Bioinformatics tools

CO 34: Learn experiments related to microbial growth and diversity

CO 35: Categorize and apply different chromatographic techniques as well as learn and remember membrane transport methods.

CO 36: Relate, predict, illustrate practical aspect of Enzymology, Biochemistry and microbial growth and diversity

CO 37: Design application aspect of Instrumentation, biostatistics and bioinformatics

Interactive Lectures

 Discussion

 Tutorials

Workshops

Self-learning assignments

Questions preparations

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

  
 

CONTENTS:

  1. Preparation of liquid and solid media for growth of microorganisms.
  2. Pure culture techniques- streak plate method, pour plate method, spread plate         method.
  3. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on         growth.
  4. Enumeration of bacteria: standard plate count.
  5. Microscopic examination of bacteria by simple staining, Gram stain, Negative staining, staining for spores etc.
  6. Hanging drop method to study motility in bacteria.
  7. study of culture characters of microorganisms
  8. Biochemical characterization of selected microbes.
  1. oxidation and fermentation tests
  2. catalase test
  3. oxidase test
  4. nitrate reduction test
  5. starch hydrolysis
  6. IMVIC
  7. H2S production test
  1. Estimation of protein in bacteria by Folin Lowry method
  2. Estimation of protein by Biuret test
  3. Estimation of reducing sugar by DNSA method
  4. Estimation of sugar in bacteria by Anthrone reagent
  5. To study the effect of substrate concentration on enzyme activity
  6. To study the effect of temperature on enzyme activity
  7. To study the effect of pH on enzyme activity
  8. To assay the activity of acid phosphatase
  9. Separation and identification of amino acids by TLC
  10. Separation of protein by SDS-PAGE
  11. Demonstrations:
    1. Gel Filtration Chromatography
    2. Ion Exchange Chromatography
    3. Adsorbtion Chromatography
    4. Affinity Chromatography
    5. Blotting Techniques
  12. Biostatistics exercises
  13. Information searching on Internet.
  14. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ

Any other practical based on theory syllabus.

Academic Year: 
2022-2023
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