LABORATORY I

Paper Code: 
CMBL 102
Credits: 
02
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course learners will:

CO 6: Remember and learn good laboratory practices

CO 7: Acquire the ability to explain principle and working of different instruments and analytical techniques.

CO 8: Acquire the ability to apply basics to solution preparation and pH estimation.

CO 9: To analyze and evaluate quantitative estimation of various bio-molecules using spectroscopy

Interactive Lectures, Discussion, Practical experiments, Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

 

 

CONTENTS

1.   Preparation of buffers

2.  To determine the pH of solutions.

3.  To prepare molar and normal solutions

4.  Verification of Beer’s law

5.  Quantitation of proteins by Biuret assay

6.  Quantitation of carbohydrates by DNS method

7.  Quantitation of cholesterol

8.  Separation of given amino acid mixture by Course chromatography

9.  Separation of sugars by thin layer chromatography

10. To study the working of light microscope

 

Academic Year: