LABORATORY I

Paper Code:

CBTE 102

Credits:

Contact Hours:

Objective:

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learners will be able to:

CO6: Remember and learn good laboratory practices

CO7: Acquire the ability to explain principle and working of different instruments and analytical techniques.

CO8: Acquire the ability to apply basics to solution preparation and pH estimation.

CO9: To analyze and evaluate quantitative estimation of various bio-molecules using spectroscopy

Interactive Lectures, Discussion, Practical experiments, Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

1. Preparation of buffers

2. To determine the pH of solutions.

3. To prepare molar and normal solutions

4. Verification of Beer’s law

5. Quantitation of proteins by Biuret assay

6. Quantitation of carbohydrates by DNS method

7. Quantitation of cholesterol

8. Separation of given amino acid mixture by Course chromatography

9. Separation of sugars by thin layer chromatography

10. To study the working of light microscope

Academic Year:

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