Laboratory II

Paper Code: 
MBL 203
Credits: 
02
Contact Hours: 
60
Objective: 

This paper will enable students to understand –

 

  1. Learn the principle and working of different instruments.
  2. Learn good laboratory practices.
  3. Qualitative and quantitative estimation of various biomolecules.

Course Outcomes (COs):

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 203

Laboratory II

CO 1 Properly use aseptic techniques, including sterilization.

CO 2 To learn the different types of microbiological media and protocols

CO 3 Know General bacteriology and microbial techniques for isolation of pure cultures of bacteria.

CO 4 To learn different staining techniques to study the various aspects of bacteria

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

 

CONTENTS

 

  1. Introduction to Microbiology laboratory, safety measures and aseptic techniques.
  2. Introduction to microscope and micrometry
  3. Study of bacterial morphology.
  4. Differential staining (Grams staining)
  5. Negative staining.
  6. Endospore staining.
  7. Media preparation
  8. Pure culture techniques- isolation of microorganisms by streak plate spread plate and pour plate method.
  9. Study of growth of bacteria
  10.  Haemocytometer

 

Academic Year: 
2020-2021
  • Printer-friendly version
  • PDF version