LABORATORY II

1.     Isolation of antibiotic resistant microorganisms by replica plating.

2.     Isolation of antibiotic resistant microorganisms by Gradient plate technique.

3.     Effect of U.V. rays on bacteria.

4.     Isolation of plasmid DNA 

5.     Isolation of genomic DNA

6.     Spectrophotometric quantitation of  nucleic acids

7.     Ouchterlony double diffusion

8.      ELISA

9.     Demonstrations:

a. Sandwich ELISA

b.Latex agglutination

c. Rocket Immunoelectrophoresis

10.  Restriction digestion of λDNA

11.  Ligation

12.  Transformation of DH5α (including preparation of competent cells)

13.  Demonstartions:

a.   Conjugation

b.  Reporter gene assay (GFP)/ Bacterial Transduction

14.  Study of various symptoms produced in plants due to virus infection.

15.  Study of viral diseases of plants/animals/human (Specimen/photographs)

16.  Different type of viruses (Photographs/sketches).

17.  Titration of Phages

18.  Isolation and identification of fungi.

19.  study of permanent slides of algae and fungi 

20.  Isolation and identification of Rhizobium from root nodules of leguminous plants.

21.  Isolation and identification of Azotobacter from soil.