Laboratory II

Paper Code: 
BTE 203
Credits: 
02
Contact Hours: 
60
Objective: 

This course will enable the students to –

 

  1. Understand the importance of aspetic conditions in laboratory
  2. To learn various culture techniqies

 

Course Outcomes (COs):

 

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessm ent Strategie

s

Paper Code

Paper Title

 

 

 

 

 

 

 

BTE 203

 

 

 

 

 

 

 

Laboratory II

CO 25 Properly use aseptic techniques, including sterilization.

 

CO 26 To learn the different types of microbiological media and protocols

 

CO 27 Know General bacteriology and microbial techniques for isolation of pure cultures of bacteria.

Approach in teaching: Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students: Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

C A test, Semester end examinatio ns, Viva Voce, Practical record book, Assignment s, Classroom interaction

 

 

CO 28 To learn different membrane transport methods.

 

 

 

CONTENTS

  1. Introduction to Microbiology laboratory, safety measures and aseptic techniques
  2. Pure culture techniques
  3. Isolation of microorganisms
  4. Simple Staining
  5. Negative staining
  6. Differential staining
  7. Identification of Fungi
  8. Media preparation
  9. Cell division: study of mitosis in onion root tip
  10. Demonstration of plasmolysis
  11. Hemocytomery
  12. Use of colorimeter to study bacterial Growth curve

 

Academic Year: 
2021-2022
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