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Laboratory-II
Paper Code:
BTE 226
Credits:
08
Contact Hours:
240
Objective:
Course Objectives:
This course will enable the students to -
- Make the students learn the practical aspect of Genetics, Molecular Biology and Immunology
- Make students learn about the various aspects of Plant tissue Culture and applications.
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BTE 226
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Laboratory-II
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Upon completion of the course students will be able to: CO 49. Learn experiments related to Molecular Biology and Genetics. CO 50. Learn demonstrations and experiments related to Immunology CO 51. Learn experiments related to Plant tissue Culture and applications |
Approach in teaching: Discussion, Tutorials Learning activities for the students: Effective questions, Simulation, Giving tasks, Practical
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Continuous Assessment Semester End Exam |
CONTENTS
- Study of mitosis and meiosis.
- X – Chromosome detection through Barr body experiment.
- To study mitosis in onion root tips
- To study meiosis in onion buds
- Isolation of plasmid DNA
- Isolation of genomic DNA
- Spectrophotometric quantitation of nucleic acids
- Demonstration of lac operon
- Blood film preparation and identification of cells.
- Ouchterlony double diffusion
- Dot ELISA
- Demonstrations:
- Sandwich ELISA
- Latex agglutination
- Rocket Immunoelectrophoresis
- Restriction digestion of λDNA
- Ligation
- Transformation of DH5α (including preparation of competent cells)
- Demonstartions:
- Conjugation
- Reporter gene assay (GFP)
- Preparation of media.
- Explant inoculation
- Callus induction
- Protoplast isolation and fusion
- Synthetic seed preparation
- Study of mutagens by Ames test
- Blood Film preparation and identification of cells
Any other practical based on theory syllabus
Academic Year:
