Laboratory II

Paper Code: 
MBL 203
Credits: 
02
Contact Hours: 
60
Objective: 

This paper will enable students to understand –

 

  1. Learn the principle and working of different instruments.
  2. Learn good laboratory practices.
  3. Qualitative and quantitative estimation of various biomolecules.

 

Course Outcomes (COs):

 

 

Course

Learning outcome (at course level)

Learning                 and teaching strategies

Assessment Strategies

Paper

Cod e

Paper Title

 

 

CO 29

Approach         in

C     A     test,

 

 

Properly use aseptic

teaching:

Semester end

 

 

techniques, including

Interactive

examinations,

 

 

sterilization.

Lectures,

Viva       Voce,

 

 

CO 30

Discussion,

Practical

 

 

To        learn        the

Practical

record    book,

 

 

different     types                 of microbiological

experiments

Assignments, Classroom

MB L 203

Labor atory II

media and protocols

CO 31

Know                  General bacteriology                       and

Learning activities for the students:

Self        learning

interaction

 

 

microbial techniques

assignments,

 

 

 

for isolation of pure

Effective

 

 

 

cultures of bacteria.

questions,

 

 

 

CO 32

Practical

 

 

 

To    learn    different

experiments     in

 

 

 

staining     techniques

lab, Giving tasks.

 

 

 

to study the various

 

 

 

 

aspects of bacteria

 

 

 

CONTENTS

  1. Introduction to Microbiology laboratory, safety measures and aseptic techniques.
  2. Introduction to microscope and micrometry
  3. Study of bacterial morphology.
  4. Differential staining (Grams staining)
  5. Negative staining.
  6. Endospore staining.
  7. Media preparation
  8. Pure culture techniques- isolation of microorganisms by streak plate spread plate and pour plate method.
  9. Study of growth of bacteria
  10. Haemocytometer

 

Academic Year: