Laboratory II

Paper Code: 
MBL 226
Credits: 
08
Contact Hours: 
240
Objective: 

Course Objectives:

This course will enable the students to –

  1. Make the students learn the practical aspect of  Genetics, Molecular Biology and Immunology
  2. Make students learn about the various aspects of Virology, Mycology, Phycology and applications.

 

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 226

 

Laboratory-II

 

Upon completion of the course students will be able to

CO 51: Learn experiments related to Molecular biology and Genetics.

CO 52: Learn demonstrations and experiments related to Immunology

CO 53: Learn experiments related to virology, mycology and phycology

Approach in teaching:

Discussion, Tutorials, Demonstration,

Learning activities for the students:

Effective questions, Simulation, Giving tasks,  Practical

Continuous Assessment

Semester End Exam

 

CONTENTS

 

  1. Isolation of antibiotic resistant microorganisms by replica plating.
  2. Isolation of antibiotic resistant microorganisms by Gradient plate technique.
  3. Effect of U.V. rays on bacteria.
  4. Isolation of plasmid DNA  
  5. Isolation of genomic DNA
  6. Spectrophotometric quantitation of  nucleic acids
  7. Ouchterlony double diffusion
  8.  ELISA
  9. Demonstrations:
    1. Sandwich ELISA
    2. Latex agglutination
    3. Rocket Immunoelectrophoresis
  10. Restriction digestion of λDNA
  11. Ligation
  12. Transformation of DH5α (including preparation of competent cells)
  13. Demonstartions:
  1. Conjugation
  2.   Reporter gene assay (GFP)/ Bacterial Transduction
  1. Study of various symptoms produced in plants due to virus infection.
  2. Study of viral diseases of plants/animals/human (Specimen/photographs)
  3. Different type of viruses (Photographs/sketches).
  4. Titration of Phages
  5. Isolation and identification of fungi.
  6. study of permanent slides of algae and fungi 
  7. Isolation and identification of Rhizobium from root nodules of leguminous plants.
  8. Isolation and identification of Azotobacter from soil.
Academic Year: 
2021-2022
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