LABORATORY-II

Paper Code: 
MBL 203
Credits: 
03
Contact Hours: 
45
Objective: 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course  learner will be able to:

CO 27: evaluate the different types of aseptic methods.

CO 28: identify the morphology of bacteria by different staining methods and establish their Grams nature.

CO 29: isolate bacteria by use of different pure culture techniques.

CO 30:  analyze the bacterial growth by spectrophotometer.

Interactive Lectures,

Discussion,

Tutorials,

Demonstrations,

Assignments

Reading JOURNALS:

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

CONTENTS

  1. Introduction to Microbiology laboratory, safety measures and aseptic techniques.
  2. Introduction to microscope and micrometry
  3. Study of bacterial morphology by simple staining.
  4. Differential staining (Grams staining)
  5. Negative staining.
  6. Endospore staining.
  7. Media preparation
  8. Pure culture techniques- isolation of microorganisms by streak plate spread plate and pour plate method.
  9. Study of growth of bacteria
  10. Haemocytometer

 

Academic Year: 
2022-2023
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