LABORATORY II

Paper Code: 
BTE 203
Credits: 
3
Contact Hours: 
60
Objective: 

Upon completion of the course learners will be able to:

CO 27: Learn, explain, evaluate and apply aseptic techniques, including sterilization.

CO 28: Remember, describe, differentiate and plan the use of  different types of microbiological media and protocols

CO 29:  Relate, outline, categorize and decide bacteriology and microbial techniques for isolation of pure cultures of bacteria.

CO 30: Tell, illustrate and imagine different membrane transport methods.

  1. Introduction to Microbiology laboratory, safety measures and aseptic techniques
  2. Pure culture techniques
  3. Isolation of microorganisms
  4. Simple Staining
  5. Negative staining
  6. Differential staining
  7. Identification of Fungi
  8. Media preparation
  9. Cell division: study of mitosis in onion root tip
  10. Demonstration of plasmolysis
  11. Hemocytomery
  12. Use of colorimeter to study bacterial Growth curve

 

 

 

Academic Year: