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LABORATORY II
Paper Code:
MBL 226
Credits:
08
Contact Hours:
240
Objective:
Course Outcomes (COs):
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Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO 68: Learn experiments related to Molecular Biology, Microbial Genetics and Genetic Engineering. CO 69: Learn demonstrations and experiments related to Immunology CO 70: Learn experiments related to Virology, Mycology and Phycology |
Discussion, Tutorials Effective questions, Simulation, Giving tasks, Practical
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Continuous Assessment Semester End Exam |
CONTENTS:
- Isolation of antibiotic resistant microorganisms by replica plating.
- Isolation of antibiotic resistant microorganisms by Gradient plate technique.
- Effect of U.V. rays on bacteria.
- Isolation of plasmid DNA
- Isolation of genomic DNA
- Spectrophotometric quantitation of nucleic acids
- Ouchterlony double diffusion
- ELISA
- Demonstrations:
- Sandwich ELISA
- Latex agglutination
- Rocket Immunoelectrophoresis
- Restriction digestion of λDNA
- Ligation
- Transformation of DH5α (including preparation of competent cells)
- Demonstartions
- Conjugation
- Reporter gene assay (GFP)/ Bacterial Transduction
- Study of various symptoms produced in plants due to virus infection.
- Study of viral diseases of plants/animals/human (Specimen/photographs)
- Different type of viruses (Photographs/sketches).
- Titration of Phages
- Isolation and identification of fungi.
- study of permanent slides of algae and fungi
- Isolation and identification of Rhizobium from root nodules of leguminous plants.
- Isolation and identification of Azotobacter from soil.
- Experimental verification that absorption at OD260 is more for denatured DNA than native ds DNA
Academic Year:
