LABORATORY II

Paper Code:

CBTE 202

Credits:

2

Contact Hours:

60

Objective:

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO18: Solve problems based on Mendel’s laws and gene interactions.

CO19 : Isolate mutants by replica plating and gradient plate techniques.

CO20 : Isolate and estimate nucleic acids from prokaryotic and eukaryotic cells.

Hands-on practical,

Interactive Lectures,

Discussion,

Demonstrations

Class test, Semester end examinations, Hands-on practical assessment

1. Isolation of mutants by replica plating.

2. Isolation of mutants by gradient plate technique.

3. Simple problems based on Mendel’s laws and gene interactions.

4. Isolation of genomic DNA from bacterial cell.

5. Isolation of DNA from plant cell.

6. Estimation of DNA by Gel electrophoresis

7. Determination of λ_max from isolated DNA

8. Isolation of RNA

Academic Year:

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