LABORATORY II

1. Study of mitosis and meiosis.
2. X – Chromosome detection through Barr body experiment.
3. To study mitosis in onion root tips
4. To study meiosis in onion buds
5. Isolation of plasmid DNA 
6. Isolation of genomic DNA
7. Spectrophotometric quantitation of  nucleic acids
8. Demonstration of lac operon
9. Blood film preparation and identification of cells.
10. Ouchterlony double diffusion
11. Dot ELISA
12. Demonstrations:
13. Sandwich ELISA
14. Latex agglutination
15. Rocket Immunoelectrophoresis
16. Restriction digestion of λDNA
17. Ligation
18. Transformation of DH5α (including preparation of competent cells)
19. Demonstartions:
20. Conjugation
21. Reporter gene assay (GFP)
22. Preparation of media.
23. Explant inoculation
24. Callus induction
25. Protoplast isolation and fusion
26. Synthetic seed preparation
27. Study of mutagens by Ames test
28. Blood Film preparation and identification of cells

Any other practical based on theory syllabus