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Laboratory II (Microbiology) (Practical)
The objective of this laboratory course is to impart practical skills in fundamental microbiological techniques, such as working in aseptic conditions, isolating and preserving microbes from various samples, identifying and counting them, and analyzing the antibiotic susceptibility of the bacteria.
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course title |
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24BTE129 |
Laboratory II (Microbiology) (Practical) |
CO49: Design experiments for isolation, identification, preservation and enumeration of microbes present in a sample. CO50: Evaluate the metabolic capability of microbes using biochemical tests. CO51: Examine the bacterial susceptibility to various antibiotics and antiseptic and disinfectant properties of various reagents. CO52: Identify and illustrate the various elements of microbiology CO53: Discuss and defend the concepts of microbiology and illustrate the exercises performed with appropriate methods and outcomes and maintain proper documentation of the same. CO54: Contribute effectively in course-specific interaction
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Approach in teaching: Hands-on practical, demonstrations, simulations
Learning activities for the students: Discussion, Tutorials, Assignments Reading Journals
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Class test, Semester end examinations, Hands-on practical assessment |
Experiments based on:
Microbiology
1. Sterilization, disinfection and safety in microbiological laboratory.
2. Preparation of media for cultivation of bacteria.
3. Isolation of bacteria in pure culture by streak plate method.
4. Maintenance of stock cultures: slants, stabs and glycerol stock cultures
5. Study of colony and growth characteristics of some common bacteria: Bacillus, E. coli, Staphylococcus, Streptococcus, etc.
6. Preparation of bacterial smear and Staining methods: Negative, Gram’s staining, endospore staining.
7. Enumeration of bacteria: standard plate count.
8. Biochemical characterization of microbes.
9. Isolation and identification of bacteria from soil/water samples.
10. Antimicrobial sensitivity test and demonstration of drug resistance.
11. Determination of phenol co-efficient of antimicrobial agents.
12. Determination of Minimum Inhibitory Concentration (MIC)
- Any other practical based on theory syllabus.
Recommended Textbooks and References:
1. Cappuccino, J. G., & Welsh, C. (2016). Microbiology: a Laboratory Manual. Benjamin-
Cummings Publishing Company.
2. Collins, C. H., Lyne, P. M., Grange, J. M., & Falkinham III, J. (2004). Collins and Lyne’s
Microbiological Methods (8th ed.). Arnolds.
3. Tille, P. M., & Forbes, B. A.(2015) . Bailey & Scott’s Diagnostic Microbiology (15th edition).
Elsevier Inc
