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Laboratory II (Practical)
Paper Code:
24CMBL202
Credits:
02
Contact Hours:
04
Objective:
This course will impart practical skills to students for isolation of mutants, extraction, estimation of nucleic acids from eukaryotic and prokaryotic cells and solve problems based on Mendel’s laws and gene interactions.
CONTENTS
1. Isolation of mutants by replica plating.
2. Isolation of mutants by gradient plate technique.
3. Simple problems based on Mendel’s laws and gene interactions.
4. Isolation of genomic DNA from bacterial cell.
5. Isolation of DNA from plant cell.
6. Estimation of DNA by Gel electrophoresis
7. Determination of λmax from isolated DNA
8. Isolation of RNA
Academic Year:
