Laboratory II

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

CONTENTS

1. Introduction to Microbiology laboratory, safety measures and aseptic techniques
2. Pure culture techniques
3. Isolation of microorganisms
4. Simple Staining
5. Negative staining
6. Differential staining
7. Identification of Fungi
8. Media preparation
9. Cell division: study of mitosis in onion root tip
10. Demonstration of plasmolysis
11. Hemocytomery
12. Use of colorimeter to study bacterial Growth curve

Laboratory II

CO 25 Properly use aseptic techniques, including sterilization.

CO 26 To learn the different types of microbiological media and protocols

CO 27 Know General bacteriology and microbial techniques for isolation of pure cultures of bacteria.

CO 28 To learn different membrane transport methods.

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction