LABORATORY III

1. Enumeration of the following in blood sample

1. 1. RBC          b.   WBC            c.   Eosinophils

2. Widal test
3. Haemagglutination test
4. Preparation of blood smear and determination of  differential WBC  count
5. Isolation and identification of Staphylococci and Streptococci from human body.
6. Separation of serum proteins by electrophoresis
7. Antibiotic sensitivity test by disc method
8. Determination of MIC of antibiotics
9. Production and estimation of Lipases etc.
10. Microbial production of antibiotics
11. Use of alginate for cell immobilization
12. Isolation of industrially important microorganisms for microbial processes
13. Isolation of Lactobacillus species from curd.
14. Production of lactic acid from whey
15. Production of baker’s yeast
16. Production of alcohol
17. Detection of microbial protease production
18. Cellulose production by microorganisms
19. Isolation of rhizospheric microflora
20. Determination of R : S ratio of soil.
21. Isolation of microorganisms from soil, water and air
22. Detection of coliforms for determination of the purity of potable water.
23. Determination of biological oxygen demand (BOD) of sewage sample.
24. Determination of chemical oxygen demand (COD) of sewage sample.
25. Isolation and identification of common microorganisms spoiling food (Fungi & bacteria).
26. Preparation of fermented foods (Saukeraut).
27. Determination of number of bacteria in milk by
    -Standard plate count
    -Direct microscopic count
28. Testing of milk by MBRT
29. Study of ammonification by bacteria
30. study of microbial antagonism and bacterial synergism
31. Estimation of nitrate in drinking water.
32. Determination of amount of CO₂ in water.
33. Isolation of xenobiotic degrading micro organisms.
34. Determination of hardness of water.
35. Antibiotic sensitivity test by disc method
36. Standard analysis of water. Determination of MPN.
37. Whole Cell immobilization

Any other practical based on theory syllabus.