Laboratory III

1. Enumeration of the following in blood sample a. RBC b. WBC c. Eosinophils 2. Widal test 3. Haemagglutination test 4. Preparation of blood smear and determination of differential WBC count 5. Isolation and identification of Staphylococci and Streptococci from human body. 6. Separation of serum proteins by electrophoresis 7. Antibiotic sensitivity test by disc method 8. Determination of MIC of antibiotics 9. Production and estimation of Lipases etc. 10. Microbial production of antibiotics 11. Use of alginate for cell immobilization 12. Isolation of industrially important microorganisms for microbial processes 13. Isolation of Lactobacillus species from curd. 14. Production of lactic acid from whey 15. Production of baker’s yeast 16. Production of alcohol 17. Detection of microbial protease production 18. Cellulose production by microorganisms 19. Isolation of rhizospheric microflora 20. Determination of R : S ratio of soil. 21. Isolation of microorganisms from soil, water and air 22. Detection of coliforms for determination of the purity of potable water. 23. Determination of biological oxygen demand (BOD) of sewage sample. 24. Determination of chemical oxygen demand (COD) of sewage sample. 25. Isolation and identification of common microorganisms spoiling food (Fungi and bacteria). 26. Preparation of fermented foods (Saukeraut). 27. Determination of number of bacteria in milk by -Standard plate count -Direct microscopic count 28. Testing of milk by MBRT 29.  Study of ammonification by bacteria 30. study of microbial antagonism and bacterial synergism.31. Estimation of nitrate in drinking water. 32. Determination of amount of CO2 in water. 33. Isolation of xenobiotic degrading micro organisms. 34. Determination of hardness of wate. 35. Antibiotic sensitivity test by disc method. 36. Standard analysis of water. Determination of MPN. 37. Whole cell immobilization.

Any other practical based on theory syllabus