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Laboratory III
This paper will enable students to understand –
- Learn the principle and working of different instruments.
- Learn good laboratory practices.
- Qualitative and quantitative estimation of various biomolecules.
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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CO 43 |
Approach in teaching: |
C A test, |
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Student will learn to culture |
Interactive Lectures, |
Semester end |
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and identify protozoa. |
Discussion, Practical |
examinations, |
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CO 44 |
experiments |
Viva Voce, |
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Student will learn to isolate |
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Practical |
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and genomic DNA, Plasmid |
Learning activities for |
record book, |
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DNA as well as RNA. |
the students: |
Assignments, |
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MBL 303 |
Laboratory III |
CO 45 Student will learn various fungal staining techniques. |
Self learning assignments, Effective questions, Practical |
Classroom interaction |
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CO 46 |
experiments in lab, |
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Student will learn to isolate |
Giving tasks. |
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and Enumerate various fungi. |
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CO 47 |
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Student will earn pure culture |
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techniques for fungi. |
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CONTENTS
- Isolation of antibiotic resistant bacteria
- Isolation of genomic DNA
- Isolation of plasmid DNA
- Isolation of RNA
- Isolation of fungi from soil
- Identification of fungi by lactophenol cotton blue staining
- Enumeration of fungal spores by haemocytometer
- Identification of Algae (permanent slides)
- Identification of protozoa (permanent slides)
- Preparation of protozoan culture
- Studies of live protozoa and preparation of slides
