LABORATORY III

Paper Code: 
MBL 303
Credits: 
03
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learners will be able to:

CO 41: learn, remember and apply technique to culture and identify protozoa.

CO 42: learn, remember and design technique t Isolate and genomic DNA, Plasmid DNA as well as RNA.

CO 43: relate and use various fungal staining techniques.

CO 44: analyze and design methode to isolate and Enumerate various fungi.

CO 45: apply pure culture techniques for isolation of bacteria.

Interactive Lectures, Discussion, Practical experiments, Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

 

 

CONTENTS

  1. Isolation of antibiotic resistant bacteria
  2. Isolation of genomic DNA
  3. Isolation of plasmid DNA
  4. Isolation of RNA
  5. Isolation of fungi from soil
  6. Identification of fungi by lactophenol cotton blue staining
  7. Enumeration of   fungal spores by haemocytometer
  8. Identification of Algae (permanent slides)
  9. Identification of protozoa (permanent slides)
  10. Preparation of protozoan culture
  11. Studies of live protozoa and preparation of slides

 

Academic Year: