LABORATORY III

Paper Code: 
CBTE 302
Credits: 
2
Contact Hours: 
60
Objective: 

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 

 

Upon completion of the course the learner will:

CO27: Apply aseptic methods to lab work

CO28: Design protocol and methodology for isolation of microbes

CO29: Isolate and identify the microorganisms producing industrially important products.

CO30: Design an experiment to enumerate the no. of CFU/ml of any sample.

CO31:   Evaluate the bacterial growth by spectrophotometer.

CO32: Analyze the principles of Bioprocess Technology

CO33: Illustrate various parts of fermenter

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

 

1.    Introduction to Microbiology laboratory, safety measures and aseptic techniques

2.    Media preparation

3.    Pure culture techniques for isolation of microorganisms

4.    Simple Staining

5.    Negative staining

6.    Differential staining

7.    Identification of Fungi

8.    Study of bacterial Growth curve

9.    Isolation of industrially important microorganisms from natural resources.

10. Study different parts of fermenter

11. Determination of Biomass Yield

12. Determination of TDT of microorganism

Academic Year: 
2023-2024
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