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LABORATORY III
Course Outcomes (COs):
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO 138: Design the experiments for isolation and screening of microbes producing commercially important products. CO 139: Apply techniques used in Bioprocess Engineering and fermentation technology CO 140: Design experiments related to Food and Dairy Microbiology CO 141: Analyze fermented food products CO 142: Design experiments to check the quality of milk. CO 143: Design experiments to determine microbial interactions and their activity in soil CO 144: Plan and apply techniques used in microbial ecology |
Hands-on practical, Interactive Lectures, Discussion, Demonstrations |
Class test, Semester end examinations, Hands-on practical assessment |
CONTENTS:
Bioprocess Engineering and Fermentation Technology
1. Basic techniques
a) Scale up from frozen vial to agar plate to shake flask culture.
b) Instrumentation: Microplate reader, spectrophotometer, microscopy.
c) Isolation of microorganisms from soil samples.
2. Experimental set-up
a) Assembly of bioreactor and sterilization.
b) Growth kinetics.
c) Substrate and product inhibitions.
d) Measurement of residual substrates.
3. Data Analysis
a) Introduction to Metabolic Flux Analysis (MFA).
4. Fermentation
a) Batch.
b) Fed-batch.
c) Continuous.
5. Unit operations
a) Microfiltrations: Separation of cells from broth.
b) Bioseparations: Various chromatographic techniques and extractions.
6. Bioanalytics
a) Analytical techniques like HPLC, FPLC, GC, GC-MS etc. for measurement
of amounts of products/substrates
7. Microbial production of antibiotics
8. Isolation of industrially important microorganisms
9. Production of alcohol
Food and Dairy Microbiology
10. Isolation and identification of common microorganisms spoiling food (Fungi & bacteria).
11.Preparation of fermented foods (Sauerkraut).
12.Enumeration of bacteria in milk samples
13.Testing of quality of milk by MBRT test
14. Determination of TDP of microorganisms
Microbial Ecology
15. Test the production of enzymes: Amylase, protease, lipase, gelatinase and cellulase by microorganisms.
16. Isolation of rhizospheric microflora and determination of R: S ratio of soil.
17. Study of ammonification by bacteria
18. Study of microbial antagonism and bacterial synergism
19. Standard analysis of water: Determination of MPN and detection of coliforms.
Any other practical based on theory syllabus.
