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Laboratory IV
This course will enable the students to –
- Understand the principle amd procedure of various immunological tests
- Learn the technique of smear preparation
- Understand the working of PCR
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BTE 403 |
Laboratory IV |
CO 54 To be aware of the important role microorganisms play in maintenance of a clean and healthy environment
CO 55 Learn various immunological techniques
CO 56 Learn smear preparation |
Approach in teaching: Interactive Lectures, Discussion, Practical experiments
Learning activities for the students: Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks. |
C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction |
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CO 57 Understand the principle underlying susceptibility |
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CO 58 Understand the working and principle of PCR. |
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CONTENTS
1. Blood Grouping and Rh typing
2. Blood film preparation and identification of cells
3. Histological examination of lymphoid tissue
4. Double diffusion
5. Radial Immuno diffusion
6. DOT- ELISA
7. Agglutination
8. Restriction Digestion of Lambda DNA
9. Demonstration of PCR
10. Isolation of plasmid DNA from bacterial cell.
