Laboratory IV

Paper Code: 
BTE 426
Credits: 
08
Contact Hours: 
240
Objective: 

Course Objectives:

This course will enable the students to –

 

  1. Make the students learn the practical aspect of animal cell culture and environmental biotechnology
  2. Make students learn about the various aspects of virology, food microbiology, bioprocesss engineering and industrial biotechnology

 

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BTE 426

Laboratory IV

Upon completion of the course students will be able to:

CO 83: Design, perform experiments, analyze and interpret data for investigating complex problems in Biotechnology and related fields.

CO 84: Learn experiments related to animal cell culture and environmental biotechnology

CO 85. Learn demonstrations and experiments related to virology and food microbiology

CO 86. Learn experiments related to bioprocesss engineering and industrial biotechnology

  Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments

Learning activities for the students:

Self learning assignments, Effective questions, Giving tasks Simulation, Practical experiments

 

Continuous Assessment

Semester End Exam

CONTENTS

  1. Introduction to Animal Cell Culture and lay out of Animal Cell Culture Laboratory
  2. Hemocytometry
  3. Cell viability
  4. Study of viral diseases of plants/animals/human (Specimen/photographs)
  5. Different type of viruses (Photographs/sketches).
  6. Phage titration
  7. Growth Kinetics
  8. Determination of Biomass Yield
  9. Wine Production.
  10. Citric Acid fermentation
  11. Determination of Thermal Death Point
  12. To test the production of enzymes: Amylases, proteases, lipases and cellulases by microorganisms.
  13. Whole Cell immobilization
  14. Isolation and identification of common microorganisms spoiling food (Fungi and bacteria).
  15. Preparation of fermented foods (Saukeraut)
  16. Determination of number of bacteria in milk by Standard plate count
  17. Testing of quality of milk sample MBRT test.
  18. Estimation of Lactic acid in curd
  19. Estimation of ascorbic acid in fruits
  20. Standard analysis of water: Determination of MPN and coliform detection in water sample.
  21. Antibiotic sensitivity test by disc method.
  22. Isolation of Xenobiont degrading  microorganisms
  23. Determination of Dissolved Oxygen concentration of water sample.
  24. Determination if Biological Oxygen Demand of sewage sample.
  25. Determination Chemical Oxygen Demand of sewage sample.
  26. Estimation of nitrate in drinking water.
  27. Determination of amount of CO2 in water.
  28. Isolation of xenobiotic degrading micro organisms.
  29. Determination of hardness of water.

Any other practical based on theory syllabus

 

 


 

 

Academic Year: 
2021-2022
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