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LABORATORY IV
Course Outcomes (COs):
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO 108: Design the experiments for isolation and screening of microbes producing commercially important products. CO 109: Analyze the quality of drinking water and milk samples using microbiological methods. CO 110: Analyze and compare the amount of lactic acid present in different brands of dairy products. CO 111: Analyze and compare the amount of Ascorbic acid present in different types of fruit/juice samples. CO 112: Apply the knowledge gained from different staining methods to identify the presence of bacteria/fungi in spoiled food samples. CO 113: Design, perform experiments, analyze and interpret data for investigating complex problems in Biotechnology and related fields. CO 114: Learn experiments related to animal cell culture and environmental biotechnology CO 115: Learn demonstrations and experiments related to virology and food microbiology CO 116: Learn experiments related to bioprocesss engineering and industrial biotechnology |
Hands-on practical, Interactive Lectures, Discussion, Demonstrations, Simulations |
Class test, Semester end examinations, Hands-on practical assessment |
1. Introduction to Animal Cell Culture and lay out of Animal Cell Culture Laboratory
2. Hemocytometry (Cell Count)
3. Cell viability
4. Cytotoxicity
5. Study of viral diseases of plants/animals/human (Specimen/photographs)
6. Different type of viruses (Photographs/sketches).
7. Phage titration
8. Growth Kinetics
9. Determination of Biomass Yield
10. Wine Production
11. Citric Acid fermentation
12. Determination of Thermal Death Point
13. To test the production of enzymes: Amylase, protease, lipase, gelatinase and cellulase by microorganisms.
14. Whole Cell immobilization
15. Isolation and identification of common microorganisms spoiling food (Fungi and bacteria).
16. Preparation of fermented foods (Saukeraut)
17. Determination of number of bacteria in milk by Standard plate count
18. Testing of quality of milk sample MBRT test.
19. Test of milk adulteration
20. Estimation of Lactic acid in curd
21. Estimation of ascorbic acid in fruits
22. Standard analysis of water: Determination of MPN and coliform detection in water sample.
23. Antibiotic sensitivity test by disc method.
24. Isolation of Xenobiotic degrading microorganisms
25. Determination of Dissolved Oxygen concentration of water sample.
26. Determination if Biological Oxygen Demand of sewage sample.
27. Determination Chemical Oxygen Demand of sewage sample.
28. Estimation of nitrate in drinking water.
29. Determination of amount of CO2 in water.
30. Determination of hardness of water.
Any other practical based on theory syllabus
