LABORATORY IV

Paper Code: 
BTE 403
Credits: 
3
Contact Hours: 
60
Objective: 

Upon completion of the course the learner will:

CO 57: Identify blood cells and lymphoid tissues by microscopic examination

CO58:  Perform various immunological techniques

CO 59: Learn to prepare blood smear

CO60: Understand the principle underlying Antibiotic susceptibility

CO61: Apply knowledge to isolate Plasmid DNA FROM bacxterial cell and its digestion

CO 62: Understand the working and principle of PCR.

  1. Blood Grouping and Rh typing
  2. Blood film preparation and identification of cells
  3. Histological examination of lymphoid tissue
  4. Double diffusion
  5. Radial Immuno diffusion
  6. DOT- ELISA
  7. Agglutination
  8. Restriction Digestion of Lambda DNA
  9. Demonstration of PCR
  10. Isolation of plasmid DNA from bacterial cell.

 

 

 

Academic Year: