This course will provide students with a comprehensive understanding of the practical aspects of the immune system, including its components and functions. Students will learn both basic and advanced techniques for detecting antigen-antibody interactions, isolating lymphocyte cells, and applying these methods in research. Additionally, the course will cover the study of normal microbial flora from different body sites, their metabolic capabilities, and the assessment of bacterial susceptibility to various antibiotics.
Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course Title |
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25MBL220 |
Laboratory IV:Immunology and Medical Microbiology (Practical)
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CO101: Prepare antigens in selected animal, collect blood, extract serum and quantify, identify, and examine the blood cells by microscopic examination. CO102: Perform antigen-antibody interactions by various immunological techniques like double diffusion, radial immunodiffusion, immunoelectrophoresis and ELISA; blotting assays to identify specific proteins or their expression CO103: Design experiments to isolate and identify normal flora from different parts of the body and analyze their metabolic capability. CO104: Examine the bacterial susceptibility to various antibiotics. CO105: Discuss and defend the concepts of immunology and medical microbiology and illustrate the exercises performed with appropriate methods and outcomes and maintain proper documentation of the same. CO106: Contribute effectively in course-specific interaction |
Approach in teaching: Hands-on practical, demonstrations, simulations
Learning activities for the students: Discussion, Tutorials, Assignments Reading Journals
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Class test, Semester end examination, Hands-on practical assessment |
Experiments based on:
Immunology
1. Selection of animals, preparation of antigens, immunization and methods of blood collection, serum separation and storage.
2. Antibody titre by ELISA method.
3. Double diffusion, Immuno-electrophoresis, Radial Immunodiffusion
4. Isolation and purification of IgG from serum or IgY from chicken egg.
5. SDS-PAGE, Immunoblotting, Dot blot assays.
6. Blood smear identification of leucocytes by Giemsa stain.
7. Separation of leucocytes by dextran method.
Medical Microbiology
8. Preparation of blood smear and Enumeration of RBC and WBC
9. Isolation of Normal Flora from Skin
10. Isolation and identification of Staphylococci and Streptococci from human body.
11. Antibiotic sensitivity test by disc method
12. Determination of MIC of antibiotics
13. Normal Flora of Oral Cavity: Dental carries susceptibility test