Laboratory IV

Paper Code: 
BTE 403
Credits: 
02
Contact Hours: 
60
Objective: 

This course will enable the students to –

 

  1.  Understand the principle amd procedure of various immunological tests
  2. Learn the technique of smear preparation
  3. Understand the working of PCR

Course Outcomes (COs): 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BTE 403

Laboratory IV

CO 54 To be aware of the important role microorganisms play in maintenance of a clean and healthy environment

CO 55 Learn various immunological techniques

CO 56 Learn smear preparation

CO 57 Understand the principle underlying susceptibility

CO 58 Understand the working and principle of PCR.

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

 

CONTENTS

  1. Blood Grouping and Rh typing
  2. Blood film preparation and identification of cells
  3. Histological examination of lymphoid tissue
  4. Double diffusion
  5. Radial Immuno diffusion
  6. DOT- ELISA
  7. Agglutination
  8. Restriction Digestion of Lambda DNA
  9. Demonstration of  PCR
  10. Isolation of plasmid DNA from bacterial cell.

 

Academic Year: 
2020-2021
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