LABORATORY V

Paper Code: 
MBL 503
Credits: 
02
Contact Hours: 
60
Objective: 

  1. This paper will enable students to understand –

     

    1. Learn the principle and working of different instruments.
    2. Learn good laboratory practices.
    3. Qualitative and quantitative estimation of various biomolecules.

Course Outcomes (COs):

 

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 503

Laboratory V

CO 68 To acquire practical skills in the technologies and methods common in Industrial microbiology.

CO 69 To apply the scientific method and hypothesis testing in the design and execution of experiments

CO 70 To understand the working and importance of fermentation.

CO 71To have a practical knowledge of Single Cell proteins in form of Mushroom Cultivation

CO 72To study the soil diversity.

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

CONTENTS

 

  1. Isolation of industrially important microorganisms
    1. amylase producing microorganism
    2. protease producing microorganism
    3. lipase producing microorganism
  2. Isolation of antibiotic producing microorganism from soil
  3. Production of alcohol.
  4. Estimation of lactic acid in curd.
  5. Estimation of ascorbic acid.
  6. Demonstration for utilization of sugars by oxidation and fermentation by microbes.
  7. Reduction of nitrate by microorganisms.
  8. Mushroom cultivation
  9. Isolation of Rhizobium from root nodules
  10. Morphology of mushrooms
Academic Year: 
2020-2021
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