Laboratory -V

Paper Code: 
BTE 503
Credits: 
2
Contact Hours: 
60
Objective: 
  1. Introduction to Plant Tissue Culture and lay out of Plant tissue culture Laboratory
  2. Aseptic manipulation techniques
  3. Sterilization of Seeds
  4. Sterilization of Buds, Leaf, Stem , Roots, Tubers, Scales
  5. Preparation of MS medium
  6. Callus Initiation from Arachis hypogea
  7. Multiple shoot proliferation in Citrus nodal segment
  8. Callus differentiation and organogenesis
  9. Introduction to Animal Cell Culture and lay out of Animal Cell Culture Laboratory
  10. Hemocytometry
  11. Cell viability
  12. Apical meristem culture to raise virus free plants
  13. Production of Rhizobium as Biofertiliser
Academic Year: 
2019-2020
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