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LABORATORY V: IMMUNOLOGY
The objectives of this laboratory course are to develop an understanding about practical aspects of components of immune system as well as their function. Basic as well as advanced methods will be taught to detect different antigen and antibody interactions, isolation of different lymphocyte cells etc. and how they can be used in respective research work.
1. Selection of animals, preparation of antigens, immunization and methods of blood collection, serum separation and storage. 2. Antibody titre by ELISA method. 3. Double diffusion, Immuno-electrophoresis, Radial Immuno diffusion 4. Complement fixation test 5. Isolation and purification of IgG from serum or IgY from chicken egg. 6. SDS-PAGE, Immunoblotting, Dot blot assays. 7. Blood smear identification of leucocytes by Giemsa stain. 8. Separation of leucocytes by dextran method. 9. Demonstration of Phagocytosis of latex beads and their cryopreservation. 10. Separation of mononuclear cells by Ficoll-Hypaque and their cryopreservation. 11. Demonstration of ELISPOT. 12. Demonstration of FACS.
